An Amperometric Biosensor for Glucose Determination Prepared from Glucose Oxidase Immobilized in Polyaniline-Polyvinylsulfonate Film
نویسندگان
چکیده
In this study, a novel amperometric glucose biosensor with immobilization of glucose oxidase on electrochemically polymerized polyaniline-polyvinylsulphonate (Pani-Pvs) films has been accomplished via the entrapment technique. Electropolymerization of aniline on the Pt surface of the Pt electrode was carried out at constant potential (0.75 V, vs. Ag/AgCl) using an electrochemical cell containing aniline and polyvinylsulphonate. Firstly, the optimum working conditions for preparing polyaniline-polyvinylsulfonate films were investigated. Determination of glucose was carried out by the oxidation of enzymatically produced H(2)O(2) at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and the optimum pH value was found to be 7.5. The storage stability and operational stability of the enzyme electrode were also studied. The results show that 75% of the response current was retained after 16 activity assays. The prepared glucose biosensor retained 80.6% of initial activity after 40 days when stored in 0.1 M phosphate buffer solution at 4 °C.
منابع مشابه
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H₂O₂ at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10-6 - 8.5×10-5 M and ...
متن کاملAn amperometric glucose biosensor based on titania sol-gel/Prussian Blue composite film.
An improved amperometric glucose biosensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared by a vapor deposition method, on a Prussian Blue (PB)-modified electrode. The method combined the merits of immobilizing biomolecules in the titania sol-gel film by vapor deposition method and the synergic catalysis effects of PB and GOD molecules. Resul...
متن کاملElectrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix
0308-8146/$ see front matter 2009 Elsevier Ltd. A doi:10.1016/j.foodchem.2009.05.087 * Corresponding author. Tel./fax: +90 232 3438624 E-mail address: [email protected] (S. Timur). A novel pyranose oxidase (PyOx) biosensor based on gold nanoparticles (AuNPs)–polyaniline(PANI)/AgCl/ gelatin nanocomposite has been developed for the glucose detection. PyOx was immobilized on the surface of glass...
متن کاملAmperometric glucose biosensor based on mediated electron transfer between immobilized glucose oxidase and plasma-polymerized thin film of dimethylaminomethylferrocene on sputtered gold electrode.
We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (approximately 20 nm), adhere...
متن کاملThe Inkjet Printing of Reducible AgNPs amperometric glucose biosensor Electrodes
The enzymes immobilization of the is crucially effective factor in biosensor preparation. Metal nanoparticles potentially able to immobilize the enzymes according to unique properties including large surface-to-volume ratio, high surface reaction activity, high catalytic efficiency, and strong adsorption ability. A novel and highly sensitive amperometric glucose biosensor was obtained by using ...
متن کامل